Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/91
Title: Prevalence Of Aflatoxlgenic Aspergillus Spp And Resistance In Groundnuts.
Authors: Dube, Mandlenkosi
Keywords: Aspergillus species
groundnuts
genetic resistance
causes of degradation
Issue Date: Jun-2014
Publisher: Lupane State University
Abstract: Aspergillus species are major causes of pre- and post-liarvest degradation in gi'oiindnnt. During 2013, the presence of aflatoxigenic Aspergillus species from gi'oundnut sold from Hwaiige, Gwanda, Umzingwane, Insiza, Beitbridge and Matobo markets was assessed. These represent areas of high groundnut production wliicli experience recurrent drought. The samples were separately analyzed for the presence of aflatoxigenic species of Aspergillus Jlaviis and A. parasiticus. The isolation of these species was carried out using dilution plating and direct plating methods respectively based on macroscopic and microscopic criteria. Altogether eight species of Aspergillus and other microbes were isolated from these samples and were identified: A. flavus, A. niger, A otyzae, A. parasiticus, A. terreus, A. tamari, A. nidullani and Rhizopus spp. However, there were variations in the degree of occurrence of each of the two species in each of these samples. The important isolates of Aspergillus jlavus and A. parasiticus were in vitro inoculated on eleven groundnut genotypes obtained from Crop Breeding Institute and Seed- Co respectively for their response to seed colonisation and infection. Only tlnee genotypes (Falcon, CG 7 and Nyanda) were found to be relatively resistant to infection and the remaining eight geonot>^es (Makulu Red, Tern, Teal, Mwenje, SC Orion, Flamingo and SC GV 00004) were susceptible in the laboratoiy tests. Overall; these results show the presence of aflatoxigenic Aspergillus spp in groundnuts in some markets. Moreover, there is limited genetic resistance to the Aspergillus flaviis and Aspergillus parasiticus in the available genotypes.
URI: http://hdl.handle.net/123456789/91
Appears in Collections:Department of Crop and Soil Science

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