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|Title:||Prevalence of aflatoxigenic aspergillus SPP and resistance in groundnut|
|Publisher:||Lupane State University|
|Abstract:||Aspergillus species are major causes of pre and post-harvest degradation in groundnut. During 2013, the presence of aflatoxigenic Aspergillus species from groundnut sold from Hwange, Gwanda, Umzingwane, Insiza, Beitbridge and Matobo markets was assessed. These represent areas of high groundnut production which experience recurrent drought. The samples were separately analyzed for the presence of aflatoxigenic species of Aspergillus flavus and A.parasiticus. The isolation of these species was carried out using dilution plating and direct plating methods respectively based on macroscopic and microscopic criteria. Altogether eight species of Aspergillus and other microbes were isolated from these samples and were identified: A.flavus, A.niger, A.oryzae, A.parasiticus, A.terreus, A.tamari, A.nidullani and Rhizopus spp. However, there were variations in the degree of occurrence of each of the two species in each of these samples. The important isolates of Aspergillus flavus and A.parasiticus were in vitro inoculated on eleven groundnut genotypes obtained from Crop Breeding Institute and Seed Co respectively for their response to seed colonization and infection. Only three genotypes (Falcon, CG7 and Nyanda) were found to be relatively resistant to infection and the remaining eight genotypes (Makulu Red, Tern, Teal, Mwenje, SC Orion, Flamingo and SC GV 00004) were susceptible in the laboratory tests. Overall, these results show the presence of aflatoxigenic Aspergillus flavus and Aspergillus parasiticus in the available genotypes|
|Appears in Collections:||Department of Crop and Soil Science|
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